cfPure™ Cell Free DNA Extraction Kit

BioChain’s cfPure™ Cell Free DNA Extraction Kit is designed for rapid and efficient purification of circulating cell free DNA (cfDNA). The system utilizes silica-coated paramagnetic particles to purify cell free DNA from less than 1 mL to greater than 10 mL of serum or plasma. The buffers were developed to ensure efficient recovery of 100 bp – 500 bp DNA fragments in order to maximize recovery of cell free DNA. The recovered DNA is suitable for a wide range of down-stream applications, such as bisulfite sequencing, NGS, and qPCR.

Features

  • Automation compatible, non-toxic chemicals
  • Fast and high cell-free DNA recovery
  • Scalable protocol accommodates sample volume inputs up to 10 ml of serum or plasma
  • Purified DNA is suitable for NGS, PCR, bisulfite sequencing, etc

cfPure™ is:

  • Cost Effective
  • Automation Friendly: BioChain experienced at automating cfPure™ on both the KingFisher™ and on pipette-based instruments – and are happy to share tips.
  • Versatile: Bead based system is amenable to extraction from <1mL to >10mL serum or plasma – in single tubes or 24- to 96-well plates.
  • Reproducible: The beads, chemistry, and protocols are optimized for consistent results.
  • Pure: Extracted cell free DNA is suitable for NGS, qPCR and other demanding applications.

Rapid and Efficient Magnetic Bead-Based Protocol

The cfPure™ protocol is rapid and easy, allowing users to process several samples in an hour or less and making it ideal for biomarker screening. BioChain has developed its own silica-coated magnetic bead technology, which is designed to efficiently recover low molecular weight cell free DNA during isolation. In addition to being easy to perform, and easy to automate, you can use less magnetic bead solution if you are purifying small amounts of plasma – saving you money.

Figure 1. cfPure™ cell free DNA extraction kit was used to isolate DNA from 5 mL of healthy human plasma. 28.3 ng of cfDNA (150-200 bp fragments) were recovered.

First Step to Non-Invasive Disease State Detection

 
Table 1. To assess reproducibility, DNA was extracted from 1 mL of plasma from 4 different human donors in duplicate using the cfPure™ cell free DNA extraction kit.
Cell free DNA offers a library of information for the non-invasive detection of cancer and prenatal genetic testing, find out more. cfDNA is present in the human blood at varying levels, from 1 to 100 ng per ml of plasma. The size of cell free DNA is typically 100 bp to 500 bp in length (with most fragments being about 160-180 bp long), making traditional DNA extraction difficult. The key to successful analysis of cfDNA is to ensure efficient purification of smaller DNA fragments (100-500 bp). As Figure 1 shows, the cfPure™ Cell Free DNA Extraction Kit does efficiently recover these important small fragments. Lastly, while cfPure™ employs a magnetic bead-based DNA extraction method, the recovered cell free DNA performs identically in sensitive applications, such as Next Generation Sequencing, to current spin column based cfDNA kits on the market.

Similar Performance in Next Generation Sequencing (NGS) to column-based methods



Table 2. cfDNA extracted from human plasma by either the cfPure™ Cell Free DNA Extraction Kit or by a competitor's spin column-based cfDNA extraction kit were subjected, in duplicate, to next generation sequencing (NGS).

Sized for Your Needs

BioChain's optimized magnetic bead based protocol enables the user to scale the protocol for use with any sample volume from 100 µl to 10 ml with highly reproducible results (Table 1). They also offer two kit sizes to fit your needs. Their 100 ml cell free DNA extraction kit contains enough reagents to extract cfDNA from 100 ml of human plasma. The larger 250 ml cell free DNA extraction kit contains sufficient reagents to extract cfDNA from 250 ml of human plasma.

Efficient Recovery of Cell Free DNA using Kingfisher Flex


Figure 2: cfDNA was purified from 600 ul of Plasma from 5 donors using the KingFisher Flex 96 DW automated purification system with either the cfPure™ Cell Free DNA Extraction Kit or by a competitors magnetic bead-based cell free DNA extraction kit. cfDNA recovery was assessed by qPCR using primers complimentary to ALU sequence DNA.

Efficient Recovery cfDNA Relative to Competition


Table 3. cfDNA was purified manually from 2 mL of plasma from three different donors by either the cfPure™ Cell Free DNA Extraction Kit or by a competitor's magnetic bead-based cell free DNA extraction kit. DNA recovery was assessed either by a fluorescent method or by qPCR using primers complimentary to ALU sequence DNA.

Cat. No.
DescriptionAmountBrand
K5011610
cfPure Cell Free DNA Purification Kit
100 ml
BioChain
K5011625
cfPure Cell Free DNA Purification Kit
250 ml
BioChain



Source:
Website: BioChain

Related to:
Brands: BioChain
Product groups: Molecular Biology