Easy Generation of M1 and M2-Macrophages

Primary human macrophages are difficult to isolate in sufficient amounts from tissue and do not proliferate in culture. In addition, the obtained cells often exhibit significant phenotypical heterogeneity. Monocyte-derived Macrophages (MDM) provide an excellent alternative, since human blood monocytes are readily available in large numbers and can be differentiated into macrophages in vitro.

The brand new PromoCell Macrophage Generation Media were designed for the straightforward and efficient differentiation of highly pure M1- or M2-macrophages directly from PBMC as a starting material.

The picture on the right hand side shows an overview on the user-customizable M1-/M2-Macrophage differentiation process (click to enlarge). 

Key advantages of the advanced chemically defined / xeno-free formulation:

  • prior monocyte purification not necessary. Simply start with PBMC
  • tightly controlled and standardized culture environment
  • devoid of all animal component-derived stimuli
Cat. No.
DescriptionAmountBrand
C-28055
M1-Macrophage Generation Medium DXF
250 ml
PromoCell
C-28056
M2-Macrophage Generation Medium DXF
250 ml
PromoCell
C-28057
Macrophage Base Medium DXF
250 ml
PromoCell
C-28051
Monocyte Attachment Medium (Ready-to-use)
250 ml
PromoCell



Upgrade your experiments with fully functional Macrophages

In the presence of the PromoCell M1-Macrophage Generation Medium DXF (contains GM-CSF) monocytes differentiate to macrophages exhibiting a CD68+/CD80+/CD163-/low M1-like polarized phenotype, whilst the M2-Macrophage Generation Medium DXF (contains M-CSF) promotes M2-like polarized CD68+/CD80-/low/CD163+ macrophages.

Exemplary flow cytometry analysis of day ten M1- and M2-Macrophages


Macrophages are tissue-resident professional phagocytes and antigen-presenting cells (APC), which exhibit extraordinary biological plasticity and differentiate from circulating peripheral blood monocytes. They perform important active and regulatory functions in innate as well as adaptive immunity.

Activated macrophages of different phenotypes are routinely classified into M1-macrophages and M2-macrophages. The classically activated M1-macrophages comprise immune effector cells with an acute inflammatory phenotype which are highly aggressive against bacteria and produce large amounts of lymphokines. The alternatively activated, anti-inflammatory M2-macrophages can be separated into at least three subgroups with differing functions, including regulation of immunity, maintenance of tolerance and tissue repair/wound healing.



Efficient Macrophage Transfection

It is well known that macrophages are more resistant to gene transfection compared to other cell types. As professional phagocytes, macrophages are endowed with many potent enzymes that can degrade nucleic acids and make gene transfer into these cells an inefficient process. Electroporation - though bypassing the endosomal degradation pathway – shows a high level of cell death and is quite expensive. As non-proliferating end-stage cells, macrophages are also difficult to transfect using viral transduction as vectors that depend on integration into a replicative genome are rather unsuitable while other viral vectors induce potent anti-viral responses in macrophages making these vectors inappropriate for gene delivery as well.

PromoKine's PromoFectin-Macrophage has been developed for optimal transfection of the hard-to-transfect macrophages (e.g. primary human macrophages derived from blood monocytes) and macrophage-derived cell lines such as RAW 264.7 as well as dendritic cells. PromoFectin-Macrophage is a mannose-bearing variant of their standard PromoFectin reagent that binds to cells expressing mannose-specific membrane receptors - such as macrophages, monocytes and dendritic cells - leading to enhanced internalization of the DNA complexes. PromoFectin-Macrophage has extremely low cytotoxicity and is compatible with serum and antibiotics, thus eliminating the need for media changes.

Cat. No.
DescriptionAmountBrand
PK-CT-2000-MAC-10
PromoFectin-Macrophage Transfection Reagent
0,1 ml
PromoKine
PK-CT-2000-MAC-50
PromoFectin-Macrophage Transfection Reagent
0,5 ml
PromoKine



Source:
Website: PromoCell

Related to:
Brands: PromoCell, PromoKine
Product groups: (Cell) Culture, Molecular Biology