Human Primary Cells

PromoCell offers a wide variety of high quality human primary cells. The tissue used for the isolation of these cells is obtained from approved medical centers following strict ethical standards. Shortly after isolation, the cells are cryopreserved using the unique serum-free PromoCell Cryo-SFM and an optimized, computer controlled freezing profile. This allows high viability of the cells and defined culture conditions after thawing.

All PromoCell primary cells are available cryopreserved or proliferating. Each cell lot is tested for cell type specific markers, cell morphology, population doubling time, and proliferation capacity. In addition, tests for the absence of HIV 1, HIV 2, HBV, HCV, fungi, mycoplasma, and other bacteria are performed. Moreover, cell pellets prepared from released cell lots and stored in RNAlater® are available. They are useful for DNA, RNA, and protein isolation and their subsequent analysis. 


Cardiac Myocytes


Human Cardiac Myocytes (HCM) are isolated from the ventricles of the adult heart. They are qualified for in vitro research on cardiac diseases and for pharmacological studies. Unlike freshly isolated rod-shaped myocytes, cultured HCM can be used for long-term experiments like investigating the long-term effects of cytokines, mechanical strain, or cell-cell interactions, as they are prepared according to a special protocol.
Initially, the HCM act more like progenitor cells in that they are not yet fully differentiated. They express the markers of early stage differentiation such as GATA-4 and sarcomeric alpha-actin and have a high capacity for proliferation. When they are grown to confluency and cultivated for an extended period of time, the differentiation process begins. Markers of late differentiation (e.g. sarcomeric alpha-actinin, slow muscle myosin) are increased and the cells begin to form myotube-like structures.

Figure: Flow cytometric analysis of PromoCell HCM after being cultured for 60 days under confluent conditions. 96 % of the cells express sarcomeric a-actinin, a marker of late differentiation.

Cat. No.DescriptionAmountBrand
C-12811
HCM-p500.000 cellsPromoCel
C-12810
HCM-c500.000 cellsPromoCel
C-14080
HCM Pellet1 mio. cellsPromoCel



Chondrocytes


Human Chondrocytes (HCH) are isolated from normal human articular cartilage from the knee and hip joints (lot specific source information is available on request). PromoCell HCH are routinely tested for their capacity to differentiate in 3D spheroids.

The articular cartilage covers the joints between bones and contains no blood vessels, lymphatic vessels, or nerve fibers. It is composed of only one specialized cell type, the chondrocytes, which produce and maintain the extracellular matrix of cartilage, i.e. collagen (mostly type II) and proteoglycans (primarily aggrecan).

Cat. No.DescriptionAmountBrand
C-12710
HCH-c500.000 cellsPromoCel
C-12750
HCH-p500.000 cellsPromoCel
C-14070
HCH Pellet1 mio. cellsPromoCel



Endothelial Cells (Large Vessels)


The endothelium is the thin layer of endothelial cells that line the interior surface of blood and lymphatic vessels - from the aorta to the smallest capillaries. In large vessels (arteries and veins), it is enclosed by two other layers namely the tunica media and the tunica externa (see Figure).

PromoCell endothelial cells (large vessels) are available from the umbilical vein and artery, the aorta, the coronary artery, the pulmonary artery, and the saphenous vein. All endothelial cells are tested for positive expression of vWF and CD31, and the absence of smooth muscle alpha-actin.

Available endothelial cell types (large vessels):

Human Umbilical Vein Endothelial Cells (HUVEC)

Cat. No. DescriptionAmountBrand
C-12200HUVEC-c single donor500.000 cellsPromoCel
C-12250HUVEC-p single donor500.000 cellsPromoCel
C-14010HUVEC single donor Pellet1 mio. cellsPromoCel
C-12203HUVEC-c pooled500.000 cellsPromoCel
C-12253HUVEC-p pooled500.000 cellsPromoCel
C-14011HUVEC pooled Pellet1 mio. cellsPromoCel
C-12206HUVEC-c Growth Medium 2 single donor500.000 cellsPromoCel
C-12207HUVEC-p Growth Medium 2 single donor500.000 cellsPromoCel
C-14008HUVEC Growth Medium 2 single donor Pellet1 mio. cellsPromoCel
C-12208HUVEC-c Growth Medium 2 pooled500.000 cellsPromoCel
C-12209HUVEC-p Growth Medium 2 pooled500.000 cellsPromoCel
C-14009HUVEC Growth Medium 2 pooled Pellet1 mio. cellsPromoCel
C-12205HUVEC-c pre-screened500.000 cellsPromoCel
C-12255HUVEC-p pre-screened500.000 cellsPromoCel
C-14012HUVEC pre-screened Pellet1 mio. cellsPromoCel

Human Umbilical Artery Endothelial Cells (HUAEC)

Cat. No. DescriptionAmountBrand
C-12202HUAEC-c500.000 cellsPromoCel
C-12252HUAEC-p500.000 cellsPromoCel
C-14013HUAEC Pellet1 mio. cellsPromoCel

Human Aortic Endothelial Cells (HAoEC)

Cat. No. DescriptionAmountBrand
C-12271HAoEC-c500.000 cellsPromoCel
C-12272HAoEC-p500.000 cellsPromoCel
C-14023HAoEC Pellet1 mio. cellsPromoCel

Human Coronary Artery Endothelial Cells (HCAEC)

Cat. No. DescriptionAmountBrand
C-12221HCAEC-c500.000 cellsPromoCel
C-12222HCAEC-p500.000 cellsPromoCel
C-14022HCAEC Pellet1 mio. cellsPromoCel

Human Pulmonary Artery Endothelial Cells (HPAEC)

Cat. No. DescriptionAmountBrand
C-12241HPAEC-c500.000 cellsPromoCel
C-12242HPAEC-p500.000 cellsPromoCel
C-14024HPAEC Pellet1 mio. cellsPromoCel

Human Saphenous Vein Endothelial Cells (HSaVEC)

Cat. No. DescriptionAmountBrand
C-12231HSaVEC-c500.000 cellsPromoCel
C-12232HSaVEC-p500.000 cellsPromoCel
C-14025HSaVEC Pellet1 mio. cellsPromoCel



Endothelial Cells (Microvascular)


The walls of capillaries are composed of a single layer of microvascular endothelial cells. This layer is so thin that molecules such as oxygen, water, and lipids can pass through it by diffusion and enter the surrounding tissues. Waste products such as carbon dioxide and urea, on the other hand, diffuse back into the blood to be carried away. Because of their location as the interface between the circulating fluid in the lumen and the surrounding tissue, microvascular endothelial cells are highly active and closely involved in numerous physiological processes.

Microvascular endothelial cells differ in morphologies and properties according to the tissues supplied by the capillaries. Therefore, PromoCell microvascular endothelial cells are available from dermal, lung, cardiac, uterine, and bladder tissues.

Available endothelial cell types (microvascular):

Human Dermal Microvascular Endothelial Cells (HDMEC)

Cat. No. DescriptionAmountBrand
C-12210HDMEC-c500.000 cellsPromoCel
C-12260HDMEC-p500.000 cellsPromoCel
C-14015HDMEC Pellet1 mio. cellsPromoCel
C-12212HDMEC-c adult500.000 cellsPromoCel
C-12262HDMEC-p adult500.000 cellsPromoCel
C-14016HDMEC adult Pellet1 mio. cellsPromoCel
C-12215HDMEC-c pre-screened500.000 cellsPromoCel
C-12265HDMEC-p pre-screened500.000 cellsPromoCel
C-14017HDMEC pre-screened Pellet1 mio. cellsPromoCel

Human Dermal Blood Endothelial Cells (HDBEC)

Cat. No. DescriptionAmountBrand
C-12211HDBEC-c500.000 cellsPromoCel
C-12214HDBEC-p500.000 cellsPromoCel
C-14018HDBEC Pellet1 mio. cellsPromoCel
C-12225HDBEC-c adult500.000 cellsPromoCel
C-12226HDBEC-p adult500.000 cellsPromoCel
C-14019HDBEC adult Pellet1 mio. cellsPromoCel

Human Dermal Lymphatic Endothelial Cells (HDLEC)

Cat. No. DescriptionAmountBrand
C-12216HDLEC-c500.000 cellsPromoCel
C-12218HDLEC-p500.000 cellsPromoCel
C-14020HDLEC Pellet1 mio. cellsPromoCel
C-12217HDLEC-c adult500.000 cellsPromoCel
C-12219HDLEC-p adult500.000 cellsPromoCel
C-14021HDLEC adult Pellet1 mio. cellsPromoCel

Human Cardiac Microvascular Endothelial Cells (HCMEC)

Cat. No. DescriptionAmountBrand
C-12285HCMEC-c500.000 cellsPromoCel
C-12286HCMEC-p500.000 cellsPromoCel
C-14029HCMEC Pellet1 mio. cellsPromoCel

Human Pulmonary Microvascular Endothelial Cells (HPMEC)

Cat. No. DescriptionAmountBrand
C-12281HPMEC-c500.000 cellsPromoCel
C-12282HPMEC-p500.000 cellsPromoCel
C-14027HPMEC Pellet1 mio. cellsPromoCel

Human Uterine Microvascular Endothelial Cells (HUtMEC)

Cat. No. DescriptionAmountBrand
C-12295HUtMEC-c500.000 cellsPromoCel
C-12296HUtMEC-p500.000 cellsPromoCel
C-14028HUtMEC Pellet1 mio. cellsPromoCel



Epithelial Cells


The epithelium is the interface between the body and the external environment. It covers all outside surfaces and inside lumina e.g. the inner lining of the respiratory tract. In addition, epithelial cells also make up exocrine and endocrine glands. The functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.

Typically, epithelial tissue is made of tightly packed cells with little intercellular space and is arranged in flat sheets forming effective barriers between the underlying tissues and the external environment.

PromoCell offers epithelial cells from different locations:
Airways: Nasal mucosa, trachea, bronchi, and distal respiratory tract
Breast: Mammary gland
Kidney: Whole kidney and renal cortex
Placenta: Amniotic membrane

Available Epithelial Cells:

Human Nasal Epithelial Cells (HNEpC)

Cat. No. DescriptionAmountBrand
C-12620HNEpC-c500.000 cellsPromoCel
C-12621HNEpC-p500.000 cellsPromoCel
C-14062HNEpC Pellet1 mio. cellsPromoCel

Human Tracheal Epithelial Cells (HTEpC)

Cat. No. DescriptionAmountBrand
C-12644HTEpC-c500.000 cellsPromoCel
C-12645HTEpC-p500.000 cellsPromoCel
C-14064HTEpC Pellet1 mio. cellsPromoCel

Human Bronchial Epithelial Cells (HBEpC)

Cat. No. DescriptionAmountBrand
C-12640HBEpC-c500.000 cellsPromoCel
C-12641HBEpC-p500.000 cellsPromoCel
C-14063HBEpC Pellet1 mio. cellsPromoCel

Human Small Airway Epithelial Cells (HSAEpC)

Cat. No. DescriptionAmountBrand
C-12642HSAEpC-c500.000 cellsPromoCel
C-12643HSAEpC-p500.000 cellsPromoCel
C-14065HSAEpC Pellet1 mio. cellsPromoCel

Human Renal Epithelial Cells (HREpC)

Cat. No. DescriptionAmountBrand
C-12665HREpC-c500.000 cellsPromoCel
C-12666HREpC-p500.000 cellsPromoCel
C-14067HREpC Pellet1 mio. cellsPromoCel

Human Renal Cortical Epithelial Cells (HRCEpC)

Cat. No. DescriptionAmountBrand
C-12660HRCEpC-c500.000 cellsPromoCel
C-12662HRCEpC-p500.000 cellsPromoCel
C-14068HRCEpC Pellet1 mio. cellsPromoCel



Fibroblasts


Fibroblasts are the most common cells in connective tissue. Their main function is to maintain the structural integrity of the connective tissue by continuously secreting extracellular matrix proteins like collagens, glycosaminoglycans, and glycoproteins. The composition of the extracellular matrix determines the physical properties of connective tissues.

Fibroblasts are morphologically heterogeneous with diverse appearances depending on their location in the body and their activity. Injury of tissue displays a proliferative stimulus for fibroblasts and induces them to produce wound healing proteins. Therefore, fibroblasts are well suited for wound healing studies.

Available Fibroblasts:

Normal Human Dermal Fibroblasts (NHDF)

Cat. No. DescriptionAmountBrand
C-12300NHDF-c500.000 cellsPromoCel
C-12350NHDF-p500.000 cellsPromoCel
C-14030NHDF Pellet1 mio. cellsPromoCel
C-12302NHDF-c adult500.000 cellsPromoCel
C-12352NHDF-p adult500.000 cellsPromoCel
C-14031NHDF adult Pellet1 mio. cellsPromoCel

Human Pulmonary Fibroblasts (HPF)

Cat. No. DescriptionAmountBrand
C-12360HPF-c500.000 cellsPromoCel
C-12361HPF-p500.000 cellsPromoCel
C-14035HPF Pellet1 mio. cellsPromoCel

Human Aortic Adventitial Fibroblasts (HAoAF)

Cat. No. DescriptionAmountBrand
C-12380HAoAF-c500.000 cellsPromoCel
C-12381HAoAF-p500.000 cellsPromoCel
C-14037HAoAF Pellet1 mio. cellsPromoCel

Human Cardiac Fibroblasts (HCF)

Cat. No. DescriptionAmountBrand
C-12375HCF-c500.000 cellsPromoCel
C-12377HCF-p500.000 cellsPromoCel
C-14036HCF Pellet1 mio. cellsPromoCel

Human Uterine Fibroblasts (HUF)

Cat. No. DescriptionAmountBrand
C-12385HUF-c500.000 cellsPromoCel
C-12386HUF-p500.000 cellsPromoCel
C-14038HUF Pellet1 mio. cellsPromoCel



Follicle Dermal Papilla Cells


Human Follicle Dermal Papilla Cells (HFDPC) are isolated from human dermis originating from the lateral scalp. Information on donor hair and skin color is available for each lot. HFDPC stain positive for alkaline phosphatase.

Dermal papillae are embedded in a laminin and collagen IV rich extracellular matrix at the base of the hair follicles. They are essential for the induction and maintenance of hair growth. Since they have androgen receptors, they can be used for in vitro screening of androgen blocking reagents.

Figure: Diagram showing the location of the dermal papilla in the hair follicle.

Cat. No.DescriptionAmountBrand
C-12071HFDPC-c500.000 cellsPromoCel
C-12072HFDPC-p500.000 cellsPromoCel
C-14005HFDPC Pellet1 mio. cellsPromoCel



Keratinocytes


Normal Human Epidermal Keratinocytes (NHEK) are available from single or from pooled donors isolated from the epidermis of juvenile foreskin or adult skin from different locations like the face, the breasts, the abdomen, and the thighs. They are the major cell type in the epidermis, making up about 90% of the cells. (Normal Human Dermal Fibroblasts (NHDF) and Normal Human Epidermal Melanocytes (NHEM) cultured in M2 medium from the same donor are available on request.)

Epidermal keratinocytes originate in the stratum basale and move up through the layers of the epidermis. During this movement, they undergo gradual differentiation and morphology changes until they reach the stratum corneum, where they form a layer of nucleus-free, flat, and highly keratinized squamous cells. This layer forms an effective barrier to the entry of infectious agents into the body and minimizes moisture loss.

Keratinocytes are also able to produce a variety of cytokines, growth factors, interleukins and complement factors. Therefore keratinocytes are important for wound healing, inflammation, and immune response.

Figure: Structure of the epidermis.

Cat. No.DescriptionAmountBrand
C-12001NHEK.f-c single donor500.000 cellsPromoCel
C-12002NHEK.f-p single donor500.000 cellsPromoCel
C-14001NHEK.f single donor Pellet1 mio. cellsPromoCel
C-12005NHEK.f-c pooled500.000 cellsPromoCel
C-12007NHEK.f-p pooled500.000 cellsPromoCel
C-14003NHEK.f pooled Pellet1 mio. cellsPromoCel
C-12003NHEK-c adult single donor500.000 cellsPromoCel
C-12004NHEK-p adult single donor500.000 cellsPromoCel
C-14002NHEK adult single donor Pellet1 mio. cellsPromoCel
C-12006NHEK-c adult pooled500.000 cellsPromoCel
C-12008NHEK-p adult pooled500.000 cellsPromoCel
C-14004NHEK adult pooled Pellet1 mio. cellsPromoCel



Melanocytes


Normal Human Epidermal Melanocytes (NHEM) are isolated from the epidermis of juvenile foreskin or adult skin from different locations including the face, the breasts, the abdomen, and the thighs. (Normal Human Epidermal Keratinocytes (NHEK) and Normal Human Dermal Fibroblasts (NHDF)  from the same donor are available on request.)

They are located in the stratum basale, but branch out between the keratinocytes in suprabasal layers. About 5-10 % of the cells in the epidermis are melanocytes.
The main purpose of melanocytes is the production of melanin, the protein responsible for the pigmentation of the skin, eyes, and hair. Melanin protects the cells in the skin and in deeper layers from the hazardous effects of UV radiation. It is produced and stored in melanosomes, which are located close to the melanocyte membrane, but it can also be transferred to neighboring keratinocytes. PromoCell melanocytes are isolated using either the serum-free, PMA (Phorbol Myristate Acetate)-containing Melanocyte Growth Medium or the serum-free and PMA-free Melanocyte Growth Medium M2. Since PMA is a tumor promoting mitogen, it can interfere with experimental approaches. Therefore, we recommend using cells isolated in Melanocyte Growth Medium M2 and also using this medium for cultivation.

Figure: Structure of the epidermis with the location of a melanocyte.

Cat. No.DescriptionAmountBrand
C-12400NHEM.f-c500.000 cellsPromoCel
C-12450NHEM.f-p500.000 cellsPromoCel
C-14040NHEM.f Pellet1 mio. cellsPromoCel
C-12402NHEM.f-c M2500.000 cellsPromoCel
C-12452NHEM.f-p M2500.000 cellsPromoCel
C-14042NHEM.f M2 Pellet1 mio. cellsPromoCel
C-12403NHEM-c M2 adult500.000 cellsPromoCel
C-12453NHEM-p M2 adult500.000 cellsPromoCel
C-14043NHEM M2 adult Pellet1 mio. cellsPromoCel



Osteoblasts


Human Osteoblasts (HOB) are isolated from femoral trabecular bone tissue from the knee or hip joint region (lot specific source information is available on request). They stain positive for alkaline phosphatase and can be mineralized using PromoCell Mineralization Medium. Osteoblasts are of mesenchymal origin and are mainly responsible for bone formation and remodeling. In vivo, they produce a collagen type I rich extracellular matrix called the osteoid.
During their development, osteoblasts undergo a maturation process called osteogenesis. When they become encased in the bone matrix, they stop proliferating and become osteocytes.

Cat. No.DescriptionAmountBrand
C-12720HOB-c500.000 cellsPromoCel
C-12760HOB-p500.000 cellsPromoCel
C-14071HOB Pellet1 mio. cellsPromoCel



Preadipocytes


Human White Preadipocytes (HWP) are isolated from adult subcutaneous or visceral adipose tissue from different locations (lot specific source information is available on request).

Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes. Since this differentiation process is controlled by a variety of growth factors and hormones, preadipocytes are suitable for the investigation of the physiological mechanisms controlling proliferation, differentiation, and function of adipose tissue.

The PromoCell adipocyte cell culture system provides preadipocytes with optimized growth media and serum-free differentiation media.

Cat. No.DescriptionAmountBrand
C-12730HWP-c subcutaneous500.000 cellsPromoCel
C-12731HWP-p subcutaneous500.000 cellsPromoCel
C-14072HWP subcutaneous Pellet1 mio. cellsPromoCel
C-12732HWP-c visceral500.000 cellsPromoCel
C-12733HWP-p visceral500.000 cellsPromoCel
C-14073HWP visceral Pellet1 mio. cellsPromoCel



Skeletal Muscle Cells


Human Skeletal Muscle Cells (SkMC) are isolated from different skeletal muscles (e.g. Musculus pectoralis major) from adult single donors (lot specific source information is available on request). They are positive for sarcomeric myosin and negative for smooth muscle specific α-actin.
New skeletal muscle cells originate from quiescent satellite cells, which are located in the muscle fibers between the basal lamina and the sarcolemma. Quiescent satellite cells are activated by stimuli such as muscle damage. After activation, the cells, now called myoblasts, start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes.

SkMC are optimal for in vitro muscle studies. They proliferate very well in the mitogen-rich PromoCell Skeletal Muscle Cell Growth Medium. Fusion to myotubes with typical multinucleated syncytia can be induced by using the PromoCell Skeletal Muscle Cell Differentiation Medium.

Cat. No. DescriptionAmountBrand
C-12530SkMC-c500.000 cellsPromoCel
C-12580SkMC-p500.000 cellsPromoCel
C-14060SkMC Pellet1 mio. cellsPromoCel



Smooth Muscle Cells


Smooth muscle cells, which form non-striated muscles, are structurally and functionally different from skeletal and cardiac muscle cells. They are located in the walls of blood vessels and hollow organs like the bladder, the uterus, and the gastrointestinal tract and are responsible for involuntary movements like peristaltic contractions, which propel food along the gastrointestinal tract.
PromoCell Smooth Muscle Cells are available from the aorta, the coronary artery, the pulmonary artery, the umbilical artery, the trachea, the bronchi, the bladder, the prostate, and the uterus.

Available Smooth Muscle Cells:

Human Aortic Smooth Muscle Cells (HAoSMC)

Cat. No. DescriptionAmountBrand
C-12532HAoSMC-p500.000 cellsPromoCel
C-12533HAoSMC-c500.000 cellsPromoCel
C-14053HAoSMC Pellet1 mio. cellsPromoCel

Human Coronary Artery Smooth Muscle Cells (HCASMC)

Cat. No. DescriptionAmountBrand
C-12511HCASMC-c500.000 cellsPromoCel
C-12512HCASMC-p500.000 cellsPromoCel
C-14052HCASMC Pellet1 mio. cellsPromoCel

Human Pulmonary Artery Smooth Muscle Cells (HPASMC)

Cat. No. DescriptionAmountBrand
C-12521HPASMC-c500.000 cellsPromoCel
C-12522HPASMC-p500.000 cellsPromoCel
C-14051HPASMC Pellet1 mio. cellsPromoCel

Human Umbilical Artery Smooth Muscle Cells (HUASMC)

Cat. No. DescriptionAmountBrand
C-12500HUASMC-c500.000 cellsPromoCel
C-12550HUASMC-p500.000 cellsPromoCel
C-14050HUASMC Pellet1 mio. cellsPromoCel

Human Tracheal Smooth Muscle Cells (HTSMC)

Cat. No. DescriptionAmountBrand
C-12565HTSMC-c500.000 cellsPromoCel
C-12566HTSMC-p500.000 cellsPromoCel
C-14056HTSMC Pellet1 mio. cellsPromoCel

Human Bronchial Smooth Muscle Cells (HBSMC)

Cat. No. DescriptionAmountBrand
C-12561HBSMC-c500.000 cellsPromoCel
C-12562HBSMC-p500.000 cellsPromoCel
C-14055HBSMC Pellet1 mio. cellsPromoCel

Human Uterine Smooth Muscle Cells (HUtSMC)

Cat. No. DescriptionAmountBrand
C-12575HUtSMC-c500.000 cellsPromoCel
C-12576HUtSMC-p500.000 cellsPromoCel
C-14059HUtSMC Pellet1 mio. cellsPromoCel



Source:
Website: PromoCell

Related to:
Brands: PromoCell
Product groups: (Cell) Culture