Inflammasome Signaling Antibodies

Inflammasomes are multi-protein complexes whose activity has been implicated in physiological and pathological inflammation. The hallmarks of inflammasome activation are the secretion of the mature forms of caspase-1 and interleukin-1β (IL-1β) from cells of the innate immune system.

An inflammasome represents a high molecular weight complex that activates inflammatory caspases and cytokines of the IL-1 family (IL-1β, IL-18 and depending on the stimulus also IL-1α). Several inflammasomes have been described which contain different sensor proteins such as NLRP1 (NALP1), NLRP3 (NALP3), IPAF (NLRC4), NLRP6 (NALP6), RIG-I and AIM-2 (absent in melanoma 2). Most of these inflammasomes require the adapter protein Asc (apoptosis-associated specklike protein containing a caspase recruitment domain) to recruit caspase-1 to the inflammasome complex. Upon binding to the inflammasome caspase-1 is cleaved and activated, leading to cleavage of its various targets and causing maturation and secretion of the pro-inflammatory IL-1β. Inflammasomes can be activated through multiple signals including live bacteria, microbial toxins, xeno-compounds, cytoplasmic pathogen-associated molecular patterns (PAMPs) and/or endogenous danger signals (DAMPs).

Inflammasome activity has been causally linked to the induction of numerous inflammatory responses, which can be either beneficial or harmful to the organism. Beneficial responses arise by maintaining homeostatic tissue function (detection and repair of tissue damages after trauma or pathogen invasion). Among the harmful inflammatory responses are particle-induced sterile inflammation, caused by host-derived particles such as monosodium urate (MSU) crystals, which are involved in the pathogenesis of gout, as well as environmental and industrial particles such as asbestos, silica and metallic nanoparticles, which include lung inflammation upon inhalation. Accumulating evidence also implicates inflammasome activity in numerous other diseases, including cancer and the development of metabolic diseases like type 2 diabetes, atherosclerosis and inflammatory bowel diseases. Beneficial effects for the host include the enhancement of vaccine efficacy.

Inflammasomes Activation & Signaling
Download the Inflammasome™ Wallchart.

Specific Caspase-1 Detection

Cat. No.
DescriptionAmountBrand
AG-20B-0044-C100
anti-Caspase-1 (p10) (mouse), mAb (Casper-2)
100 ug
AdipoGen
AG-20B-0044B-C100
anti-Caspase-1 (p10) (mouse), mAb (Casper-2) (Biotin)
100 ug
AdipoGen
AG-20B-0042-C100
anti-Caspase-1 (p20) (mouse), mAb (Casper-1)
100 ug
AdipoGen
AG-20B-0042B-C100
anti-Caspase-1 (p20) (mouse), mAb (Casper-1) (Biotin)
100 ug
AdipoGen
AG-20B-0048-C100
anti-Caspase-1 (p20) (human), mAb (Bally-1)
100 ug
AdipoGen
AG-20B-0048B-C100
anti-Caspase-1 (p20) (human), mAb (Bally-1) (Biotin)
100 ug
AdipoGen
AG-CP3-0002-M001
Z-VAD-FMK (Cell permeable) [CAS no: 187389-52-2 ]
1 mg
AdipoGen
AG-CP3-0002-M005
Z-VAD-FMK (Cell permeable) [CAS no: 187389-52-2 ]
5 mg
AdipoGen
AG-CP3-0006-M001
Q-VD-OPh [CAS no: 1135695-98-5]
1 mg
AdipoGen
AG-CP3-0006-3001
Q-VD-OPh [CAS no: 1135695-98-5]
3 x 1 mg
AdipoGen
AG-CP3-0006-M005
Q-VD-OPh [CAS no: 1135695-98-5]
5 mg
AdipoGen
AG-46B-0003-KI01
Caspase-1 (mouse) Matched Pair Detection Set
1 set
AdipoGen
AG-45B-0002-KI01
Caspase-1 (mouse) ELISA Kit
96 wells
AdipoGen

Standard Inflammasomes Signaling Antibodies

Cat. No.DescriptionAmountBrand
Nod-like Receptors (NLRs)
AG-20B-0045-C100anti-NAIP1/2/5 (mouse), MAb (Naipa-1)100 ug AdipoGen
AG-25B-0005-C100anti-NLRP1/NALP1 (human), PAb (AL176)100 ugAdipoGen
AG-20B-0014-C100anti-NLRP3/NALP3, MAb (Cryo-2)100 ugAdipoGen
AG-20B-0006-C100anti-NLRP3/NALP3 (mouse), MAb (Cryo-1)100 ugAdipoGen
AG-20B-0046-C100anti-NLRP6/NALP6 (human), MAb (Clint-1)100 ugAdipoGen
AG-25B-0021-C100anti-NLRP12/NALP12 (human), PAb (AL236)100 ugAdipoGen
AG-25B-0013-C050anti-Nod1 (human), PAb (AL184)50 ugAdipoGen
RIG-like Helicases (RLHs) – Antiviral Signaling
AG-20B-0009-C100anti-RIG-I, MAb (Alme-1)100 ugAdipoGen
AG-20B-0009B-C100anti-RIG-I, MAb (Alme-1) (Biotin)100 ugAdipoGen
AG-20B-0004-C100anti-Cardif (human), MAb (Adri-1)100 ugAdipoGen
AG-25B-0001-R100anti-MDA5 (mouse), PAb (AL180)100 ulAdipoGen
AG-20B-0013-C100anti-MDA5 (human), MAb (Hely-1)100 ugAdipoGen
AG-20B-0001-C100anti-NS3 (HCV), MAb (1B6)100 ugAdipoGen
AG-20B-0002-C100anti-NS5B (HCV), MAb (5B-3B1)100 ugAdipoGen
AG-20B-0003-C100anti-NS5B (HCV), MAb (blocking) (5B-12B7)100 ugAdipoGen
Cytosolic DNA Sensor
AG-20B-0040-C100anti-AIM2 (human), MAb (3B10)100 ugAdipoGen
Signaling Antibodies
AG-25B-0006-C100anti-Asc, PAb (AL177)100 ugAdipoGen
AG-25B-0006PF-C100anti-Asc, PAb (AL177) (preservative free)100 ugAdipoGen
AG-25B-0006TS-C100
anti-Asc, pAb (AL177) (ATTO 647N)
100 ug
AdipoGen
AG-25B-0020-C100anti-Pyrin (human) , PAb (AL196)100 ugAdipoGen
AG-25B-0011-R050anti-TRAM (human), PAb (AL239)50 ulAdipoGen
AG-25B-0008-C050anti-TRIF (human), PAb (AL227)50 ugAdipoGen
Cytosolic PAMPs Sensors
AG-20B-0060-C100anti-Caspase-4/11 (p20), MAb (Flamy-1)100 ugAdipoGen
AG-20B-0060B-C100anti-Caspase-4/11 (p20), MAb (Flamy-1) (Biotin)100 ugAdipoGen
AG-20B-0061-C100anti-Caspase-11 (p20) (mouse), MAb (Flamy-2)100 ugAdipoGen

Inflammasome Activators

Cat. No.DescriptionAmountBrand
AG-40B-0095-C100Flagellin100 ugAdipoGen
AG-40B-0025-C010Flagellin (high purity)10 ugAdipoGen
AG-40B-0125-C010Flagellin (rec.)10 ugAdipoGen
AG-40B-0126-C010Flagellin (NLRC4 Mutant) (rec.)10 ugAdipoGen
AG-40B-0127-C010Flagellin (TLR5 Mutant) (rec.)10 ugAdipoGen
AG-CR1-3950-M002Monosodium urate [MSU] (crystals) (CAS no: 1198-77-2)2 mgAdipoGen
AG-CR1-3951-M010Monosodium urate [MSU] (ready-to-use) (CAS no: 1198-77-2)10 mgAdipoGen
AG-CN2-0020-M005Nigericin . sodium salt (CAS no: 28643-80-3)5 mgAdipoGen
AG-CN2-0489-M250
N-Acetyl-D-glucosamine [CAS no: 7512-17-6]
250 mg
AdipoGen
AG-CR1-3582-M005R-848 (CAS no: 144875-48-9)5 mgAdipoGen

Inflammasome “Priming” Activators

Priming of the NLRP3 Inflammasome
The most prominent function of the NLRP3 inflammasome is the processing and activation of pro-interleukin-1β (pro-IL-1β). Yet most cells do not express pro-IL-1β and thus prior expression of pro-IL-1β is required. This can be achieved by stimulating receptors such as TLRs (e.g. LPS), NODs or TNF-Rs (e.g. TNF-α) that activate NF-κB and initiate pro-IL-1β transcription. This process of pro-IL-1β induction is called priming (Signal 1). Priming also induces NF-κB-dependent transcription of NLRP3. An additional stimulus (Signal 2) results in the activation of the NLRP3 inflammasome and subsequent initiation of downstream signaling. In the absence of priming, NLRP3 inflammasome-dependent caspase-1 activation can also be observed, but IL-1β secretion is absent.

Cat. No.DescriptionAmountBrand
AG-40B-0006-C010TNF-alpha, Soluble (human) (rec.)10 ugAdipoGen
AG-40B-0019-C010TNF-alpha (human) (multimeric) (rec.)10 ugAdipoGen
AG-40B-0021-C010
TNF-alpha (mouse) (multimeric) (rec.)
10 ug
AdipoGen
IAX-100
Lipopolysaccharides (LPS)
1 mg
Innaxon

Inflammasome Signaling-related Proteins & Antibodies

Cat. No.
DescriptionAmountBrand
AG-40B-0023-C010
IL-1beta (human) (rec.) (untagged)
10 ug
AdipoGen
AG-40B-0086-C010
IL-1beta (mouse) (rec.) (untagged)
10 ug
AdipoGen
AG-40B-0024-C050
IL-1R1 (human):Fc (human) (rec.)
50 ug
AdipoGen
AG-20B-0050-C100
anti-IL-1alpha (mouse), mAb (Bamboo-1)
100 ug
AdipoGen
AG-20B-0058-C100
anti-IL-1alpha (mouse), mAb (Bamboo-2)
100 ug
AdipoGen
AG-45B-0003-KI01
IL-1alpha (mouse) ELISA Kit
96 wells
AdipoGen
AG-46B-0004-KI01
IL-1alpha (mouse) Matched Pair Detection Set
1 set
AdipoGen
AG-20B-0064-C100
anti-IL-1alpha (p18) (mouse), mAb (Teo-1)
100 ug
AdipoGen
AG-27B-0011-C100
anti-IL-1R2 (mouse), mAb (rec.) (Praxy-1-1)
100 ug
AdipoGen

Microtubule Antibodies

Cat. No.
DescriptionAmountBrand
AG-20B-0068-C100
anti-alpha-Tubulin (acetylated), mAb (TEU318)
100 ug
AdipoGen
AG-20B-0020-C100
anti-Polyglutamylation Modification, mAb (GT335)
100 ug
AdipoGen
AG-20B-0020B-C100
anti-Polyglutamylation Modification, mAb (GT335) (Biotin)
100 ug
AdipoGen
AG-25B-0030-C050
anti-Polyglutamate chain (polyE), pAb (IN105)
50 ug
AdipoGen

Inflammasome Inhibitors

Cat. No.DescriptionAmountBrand
AG-CR1-3616-M025(R)-3-Hydroxybutyric acid [CAS no: 625-72-9]25 mgAdipoGen
AG-CR1-3617-M025(S)-3-Hydroxybutyric acid [CAS no: 6168-83-8]25 mgAdipoGen
AG-CN2-0458-M001Arglabin [CAS no: 84692-91-1]1 mgAdipoGen
AG-CR1-0013-M010BAY 11-7082 [CAS no: 19542-67-7]10 mgAdipoGen
AG-CR1-3567-M001Colcemid [CAS no: 477-30-5]1 mgAdipoGen
AG-CN2-0048-G001Colchicine [CAS no: 64-86-8]1 gAdipoGen
BVT-0447-M001Cytochalasin H [CAS no: 53760-19-3]1 mgBioViotica
AG-CR1-0045-M005Dynasore [CAS no: 304448-55-31]5 mgAdipoGen
AG-CR1-3613-G001Glyburide (USP) [CAS no: 10238-21-8]1 gAdipoGen
AG-CN2-0038-C100Ilimaquinone [CAS no: 71678-03-0]100 ugAdipoGen
AG-CN2-0459-M010Isoliquiritigenin [CAS no: 961-29-5]10 mgAdipoGen
AG-CN2-0037-C050Jasplakinolide (high purity) [CAS no: 102396-24-7]50 ugAdipoGen
AG-CN2-0027-C100Latrunculin A [CAS no: 76343-93-6]100 ugAdipoGen
AG-CN2-0031-M001Latrunculin B [CAS no: 76343-94-7]1 mgAdipoGen
AG-CR1-3615-M001MCC950 . sodium salt [CAS no: 256373-96-3]1 mgAdipoGen
AG-CR1-0019-M005Nocodazole [CAS no: 31430-18-9]5 mgAdipoGen
AG-CN2-0045-M001Paclitaxel [CAS no: 33069-62-4]1 mgAdipoGen
AG-CN2-0455-M010Parthenolide [CAS no: 20554-84-1]10 mgAdipoGen
AG-CN2-0049-M100Podophyllotoxin [CAS no: 518-28-5]100 mgAdipoGen
AG-CL1-0001-M001Prostaglandin E2 [CAS no: 363-24-6]1 mgAdipoGen
AG-CN2-0083-C100Pseudolaric acid B [CAS no: 82508-31-4]100 ugAdipoGen
AG-CN2-0033-M050Resveratrol [CAS no: 501-36-0]50 mgAdipoGen
AG-CN2-0440-C500Sceptrin . dihydrochloride [CAS no: 79703-25-6]500 ugAdipoGen
AG-CN2-0035-C010Swinholide A [CAS no: 95927-67-6]10 ugAdipoGen
AG-CN2-0074-M005Thiocolchicine [CAS no: 2730-71-4]5 mgAdipoGen
AG-CN2-0454-M020Vinpocetine [CAS no: 42971-09-5]20 mgAdipoGen
AG-CN2-0490-M001Withaferin A [CAS no: 5119-48-2]1 mgAdipoGen



Source:
Brochure: Innate Immunity, Inflammasome Wallchart, Inflammasome Signaling

Related to:
Brands: AdipoGen Life Sciences, Innaxon
Product groups: Antibodies, Chemicals, Proteins / Signaling Molecules